FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n02_ALm223RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n02_ALm223RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36039283
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA38250.09.9337359
CGGGAAT80200.09.0124641
CCGGGAT105550.08.5599191
CTACCGT48200.08.4743198
CGCGGGT76500.08.2673161
CCCGTAT50750.08.15187551
CGTATCG45350.08.0653663
CGCGGAT44850.07.9519411
GCGTCGT287350.07.918591594-95
CGGGGAT132250.07.6947131
CGGGGTT109700.07.54252861
GTATCGA47250.07.53905774
GGGGAAT195950.07.49872731
GATCGGA682400.07.493740694-95
CTCGGAT100200.07.45085761
AGATCGG756050.07.307230594-95
CGTCGTG285700.07.232736694-95
ATCGGAA649050.07.201791394-95
CTGGAAT363450.07.05209971
CTCGGTT71750.06.95891761
Produced by FastQC (version 0.11.5)