FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n02_ALm222RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n02_ALm222RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42127147
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA40450.011.3897849
CGCGGGT80100.09.4991241
CGGGGAT133000.09.2249531
CTACCGT55700.08.6977568
CCGGGAT116300.08.5868851
GCGTCGT334200.07.79626994-95
AGCGTCG387600.07.500397794-95
GATCGGA789350.07.486303394-95
ATCGGAA747550.07.36160494-95
CGGGGTT138700.07.3029651
CGTCGTG324900.07.27377894-95
GGGGAAT208700.07.2460411
AGAGCGT465150.07.209884694-95
AGATCGG878150.07.170140794-95
CGGGAAT95750.07.00287631
ACTACCG73200.06.9427647
GCGGGTT72350.06.90155361
CGGGCAT166400.06.88747171
CGGCGAT74900.06.793571
CCCGGAT138150.06.78127671
Produced by FastQC (version 0.11.5)