FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n02_ALm221RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n02_ALm221RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34157646
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA32150.011.6707439
CTACCGT45300.09.4361918
CGCGGGT61050.09.0354311
CGGGGAT104500.09.0100151
CGGGGTT109800.08.53179551
CGTATCG36600.08.3064383
CCGGGAT93800.08.0606641
GGGGAAT174950.07.90961931
CGCGATT11804.9991286E-87.65681461
GTATCGA45600.07.60348944
CGCGAAT18850.07.5680931
CCCGTAT52950.07.5437951
CGACCGT27300.07.490011
CGGGAAT78800.07.48293541
GCGTCGT253600.07.42709294-95
ATCGGAA567150.07.253453794-95
TCGGATT56350.07.25210762
GATCGGA592650.07.20586894-95
AGCGTCG287850.07.08796994-95
GTCGGAT39650.07.07595971
Produced by FastQC (version 0.11.5)