FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n02_ALm216RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n02_ALm216RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34410715
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA33550.09.7681129
CGCGTAT8601.9839717E-89.4000171
CGGGGAT104950.08.7901741
CGCGGGT68500.08.5387191
CCGGGAT95900.08.1321131
CGGGGTT107000.08.0884591
GGGGAAT175400.08.0791341
CTACCGT45800.07.67397368
CGTATCG36500.07.54815773
GCGTCGT268500.07.49262394-95
GATCGGA640000.07.455807794-95
AGCGTCG316350.07.440473694-95
CTCGGAT93550.07.3705921
ATCGGAA606900.07.31845294-95
CCCGTAT50200.07.293991
AGATCGG720200.07.262045494-95
GGGAAAT277550.07.19591861
CGTCGTG264750.07.150182294-95
CGGGAAT80700.07.13000731
CGGGTAT35050.07.05494261
Produced by FastQC (version 0.11.5)