FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n02_ALm215RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n02_ALm215RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32166708
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA30500.09.6550219
CGGGGAT103300.09.2529291
CGCGGGT61700.08.6320981
GCGTCGT254000.08.26620794-95
GGGGAAT164050.08.0004851
CCGGGAT94950.07.86299751
CGGGGTT97850.07.77575641
CGGGAAT72300.07.6296251
CTCGGAT89300.07.56171561
GTCGGAT34500.07.4431741
CGTCGTG251200.07.441197494-95
GATCGGA595750.07.41942194-95
CGACCGT27600.07.4087151
CTACCGT42000.07.35064468
ATCGGAA566600.07.331637494-95
AGCGTCG297000.07.2213694-95
GTTCGAT38250.07.21074341
AGATCGG667750.07.11739294-95
AGAGCGT351150.07.081768594-95
CGGGTAT32850.06.9484731
Produced by FastQC (version 0.11.5)