FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n02_ALm214RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n02_ALm214RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36535858
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT67600.09.0044341
GGGGAAT180000.08.6390971
CGGGGAT118750.08.6098981
TACCGTA35700.08.3816999
CGGGGTT112800.08.2208851
CCGGGAT101650.07.90628531
CGCGAAT21750.07.8711171
GCGTCGT286850.07.81633294-95
CGGGTGT111050.07.70809561
GATCGGA688050.07.39064894-95
CGTCGTG283850.07.371788594-95
CGCGGAT46900.07.3005031
ATCGGAA651400.07.25589894-95
AGATCGG769900.07.23117994-95
AGCGTCG330850.07.193205494-95
CTCGGAT102550.07.1413151
CGGGTCT133200.07.1323553
CTACCGT44350.07.06821878
AGAGCGT400800.07.02817994-95
CGTATCG39050.06.9336363
Produced by FastQC (version 0.11.5)