FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n02_ALm213RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n02_ALm213RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33460564
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA32150.011.6708359
CGCGGGT60200.010.1111481
CTACCGT41400.08.8337458
GGGGAAT165400.08.7402741
CGCGATT12753.4742698E-108.2053951
CTCGGAT94400.08.11037351
CGGGGAT106800.07.9256651
GCGTCGT274550.07.82056994-95
CCGGGAT90150.07.7542291
CGGGAAT73850.07.72712141
CGGGGTT100700.07.5085251
CGGGATT56400.07.4197721
GATCGGA640050.07.35497494-95
CGTCGTG268350.07.346286394-95
CTCGGTT63750.07.3102611
CGACCGT27400.07.2892981
CCCGGAT110100.07.2561951
AGCGTCG314500.07.18964894-95
AGATCGG714050.07.06840994-95
ATCGGAA608400.06.980228494-95
Produced by FastQC (version 0.11.5)