FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n02_ALm208RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n02_ALm208RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37869030
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA38600.010.2128249
CGCGGGT74350.08.50672151
CGGGGAT119700.08.5018051
CTACCGT52700.08.1112348
CCGGGAT107950.08.0175241
CGGGGTT116850.08.0173171
CCCGTAT56600.07.81372361
CTCGGAT105400.07.76031541
ATCGGAA705550.07.732591694-95
GCGTCGT308150.07.69237194-95
GGGGAAT186200.07.6363011
GATCGGA746450.07.63345994-95
CGCGAAT22050.07.5483321
CGCGATT15202.0372681E-107.5086041
AGCGTCG355350.07.432592494-95
CGTCGTG304450.07.356776794-95
CGACCTA23750.07.1994564
AGATCGG836200.07.14080294-95
AGAGCGT427700.07.10824894-95
TCGGAAG697100.06.967710594-95
Produced by FastQC (version 0.11.5)