FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n02_ALm207RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n02_ALm207RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36630626
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA35350.09.8082649
GGGGAAT185200.08.9619871
CGCGGGT70400.08.4441751
GCGTCGT325300.08.40380694-95
CTACCGT44900.08.1452258
CGGGGAT113150.08.069871
CGCGGAT48200.07.9920441
CGCGAAT21500.07.9631321
AGCGTCG375200.07.77989694-95
CGTCGTG315500.07.746414794-95
GATCGGA774900.07.67184794-95
CTCGTTT76550.07.51726771
CTCGGTT64100.07.41928151
CGGGGTT115050.07.39922571
ATCGGAA730400.07.33281194-95
AGATCGG867600.07.331211694-95
AGAGCGT449850.07.30195594-95
GTCGGAT37300.07.2675141
CGGGAAT83850.07.25983521
CTCGGAT103550.07.164641
Produced by FastQC (version 0.11.5)