FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n02_ALm206RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n02_ALm206RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39208073
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT76450.09.89073851
CGGGGAT121350.08.5433031
GCGTCGT373050.08.49973994-95
TACCGTA36350.08.362389
CGGGGTT117900.08.1479181
CTACCGT51900.08.1447358
CCGGGAT111250.08.1219941
CGGGATT69800.07.971491
GGGGAAT198600.07.73450141
GATCGGA913150.07.68611294-95
AGCGTCG425400.07.638005794-95
CGTCGTG362150.07.634064794-95
CGGGTAT41900.07.6044861
GTCGGAT45950.07.55520771
AGAGCGT520950.07.52735594-95
CGTATCG41800.07.5003073
ATCGGAA857150.07.495520694-95
AGATCGG1019350.07.286113394-95
CGCGGAT52300.07.2744031
CGGGAAT90700.07.13084031
Produced by FastQC (version 0.11.5)