FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n02_ALm205RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n02_ALm205RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32133991
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA31600.010.8219349
CTACCGT42350.09.5328698
CGCGGGT61500.08.9699231
CGCGTAT7804.5240267E-68.5357021
CCGGGAT86500.08.0268031
CGACCTA18450.07.98047734
CGGGGAT97250.07.92193461
GCGTCGT275950.07.815325794-95
AGAGCGT390250.07.55301894-95
CGACCGT26550.07.52299171
GATCGGA664700.07.453843694-95
AGCGTCG317500.07.443393794-95
ATCGGAA630200.07.41717294-95
GGGGAAT164650.07.2785461
CGGGGTT101750.07.24441151
AGATCGG744800.07.235798494-95
CGTCGTG270250.07.180389494-95
AAGAGCG461850.07.086632794-95
ACTACCG54300.07.08503447
GAGCGTC384550.07.028798694-95
Produced by FastQC (version 0.11.5)