FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24833229
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2685751.0815146109271574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA23150.017.22600436-37
TAATGCG24050.016.07894734-35
ACGTCTG125700.015.335114514-15
CGTCTGA133100.014.96420116-17
AGAGCAC272850.014.8038198
CTCGTAT36800.014.46457644-45
CACGTCT138400.014.35620614-15
TCGGAAG282200.014.1290463
CGGAAGA290350.014.0425264
TCTCGTA43500.013.98485542-43
ACACGTC140700.013.95138512-13
ATCGGAA290900.013.905422
CCGCGAA32100.013.52480834-35
GATCGGA290550.013.2989841
CACACGT155200.012.93816712-13
TCCGCGA32350.012.46693534-35
GAGCACA351200.011.8657969
GCGAAAT36150.011.81926436-37
ATCTCGT49550.011.50998742-43
CTACCGT18800.011.347838
Produced by FastQC (version 0.11.5)