FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n01_ALm232RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n01_ALm232RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33947211
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA158400.038.66809536-37
TCCGCGA186650.033.5917434-35
CCGCGAA183800.033.15642534-35
GCGAAAT188850.031.06250836-37
AATCGCG33000.030.73078740-41
ACTCCGC220150.028.80375732-33
CGCGTAT14950.026.21210344-45
CGAAATC155600.026.14613538-39
CTCCGCG241900.025.66411432-33
CACTCCG254450.024.4170130-31
ATCGCGT26600.021.24981142-43
GTCACTC309050.020.41063528-29
GAGCACA641450.019.6602449
CGTCTGA340800.018.89999616-17
CGAAATG99800.018.41917638-39
AGAGCAC688500.018.35138
ACGTCTG347400.018.0486914-15
CGGAAGA702050.017.9984594
TCGGAAG697850.017.9774483
ACACGTC358100.017.51588612-13
Produced by FastQC (version 0.11.5)