FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n01_ALm231RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n01_ALm231RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32679039
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA173050.039.27841636-37
CCGCGAA195250.034.3623934-35
TCCGCGA204200.033.9379434-35
GCGAAAT198750.032.72960736-37
AATCGCG32650.031.42392740-41
ACTCCGC227150.030.59272632-33
CTCCGCG254350.026.83561932-33
CGAAATC173700.026.38864538-39
CACTCCG259800.026.16303330-31
ATCGCGT23300.024.97296342-43
CGCGTAT14900.023.59040544-45
GTCACTC330650.021.02384228-29
GAGCACA688200.020.6990179
TCGCGTA18450.019.69495442-43
CGTCTGA363350.019.63572516-17
AGAGCAC721450.019.6133988
CGAAATG100350.019.45422738-39
ACGTCTG367250.018.83872814-15
CGGAAGA740600.018.8187834
AATCGGG69350.018.62997840-41
Produced by FastQC (version 0.11.5)