FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n01_ALm230RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n01_ALm230RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41051247
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA139550.033.90131836-37
CCGCGAA172850.027.20526334-35
GCGAAAT169100.027.16251636-37
TCCGCGA181250.026.65201434-35
AATCGCG29600.024.39170640-41
ACTCCGC207300.023.80695732-33
CGAAATC147350.022.12996138-39
CACTCCG228500.021.0369130-31
CTCCGCG234300.020.64792432-33
ATCGCGT23550.015.9340642-43
GTCACTC344950.014.55481628-29
CGCGTAT14400.014.51377844-45
GAGCACA751350.013.5730319
TCACTCC379550.013.41570230-31
ACGTCTG369450.013.31995714-15
CGAAATG104900.013.10881338-39
CGTCTGA394450.012.90926616-17
AGAGCAC790150.012.8043868
CACGTCT401850.012.65381214-15
ACACGTC388050.012.62638412-13
Produced by FastQC (version 0.11.5)