FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n01_ALm229RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n01_ALm229RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30325309
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA101800.033.431536-37
GCGAAAT119900.027.84990136-37
CCGCGAA124200.027.57408534-35
TCCGCGA132900.026.48382634-35
AATCGCG20750.023.92154540-41
ACTCCGC150250.023.5520832-33
CGAAATC106100.021.91433738-39
CACTCCG166200.020.99176630-31
CTCCGCG178750.019.63748432-33
CGCGTAT9800.016.47949644-45
ATCGCGT18200.014.876286542-43
GTCACTC244500.014.72578728-29
TCACTCC272150.013.36057230-31
GAGCACA545400.013.3336269
ACGTCTG265050.013.306746514-15
AGAGCAC565350.012.88848
CGTCTGA278650.012.83630116-17
ACACGTC279000.012.63285512-13
CACGTCT287650.012.51722514-15
TACCGTA33600.012.4403219
Produced by FastQC (version 0.11.5)