FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n01_ALm224RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n01_ALm224RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34525370
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT426250.1234599368522336TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC90400.030.89571646-47
TCGTATG88850.030.57950844-45
TATGCCG95900.028.90093648-49
CTCGTAT87050.027.80144344-45
TCTCGTA106400.021.04896442-43
CGGCTAT148200.019.39075334-35
CCGGCTA148600.018.89106832-33
ATCTCGT125500.017.9968942-43
ATGCCGT157100.017.55156148-49
TCACCGG168350.017.0840230-31
GTATGCC165050.017.03709646-47
ACCGGCT171050.016.77264832-33
GTCACCG176350.016.05314628-29
CCGTCTT169100.015.87114852-53
GCCGTCT167500.015.83796250-51
TATGATC160600.014.80295938-39
TGCCGTC194500.014.640655550-51
TACCGTA34700.013.004129
CGTCTTC205150.012.76960752-53
GCTATGA244700.012.06409236-37
Produced by FastQC (version 0.11.5)