FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n01_ALm223RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n01_ALm223RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36039283
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT495810.13757487905627866TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC105050.031.78676246-47
TCGTATG105550.030.98378844-45
TATGCCG108550.030.58682448-49
CTCGTAT102700.029.1148244-45
TCTCGTA128500.022.08634842-43
CGGCTAT167000.020.73468634-35
ATCTCGT139450.020.19878842-43
CCGGCTA170800.020.00919732-33
ATGCCGT170750.019.47261848-49
GTATGCC179350.018.75076146-47
ACCGGCT190600.018.1424232-33
GCCGTCT176800.017.98690850-51
GTCACCG199050.017.30072628-29
TCACCGG205550.016.88068430-31
CCGTCTT191650.016.54398252-53
TGCCGTC206600.016.0477250-51
TATGATC185250.015.58957138-39
GATCTCG202300.014.02915340-41
CGTCTTC235850.013.32267852-53
CACCGGC272150.012.90675730-31
Produced by FastQC (version 0.11.5)