FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n01_ALm222RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n01_ALm222RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42127147
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT624890.14833427955612566TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC128800.032.379346-47
TCGTATG130200.031.33809344-45
TATGCCG133750.030.5772348-49
CTCGTAT124350.029.22173344-45
TCTCGTA146650.023.14245642-43
CGGCTAT199700.021.45434234-35
ATCTCGT167350.020.42181842-43
CCGGCTA207700.020.17063732-33
ATGCCGT204000.019.91951248-49
GTATGCC215450.019.33489846-47
ACCGGCT226300.019.0480132-33
TCACCGG230300.018.71723730-31
GCCGTCT218100.017.7388350-51
GTCACCG241400.017.40407428-29
CCGTCTT225950.017.3646952-53
TGCCGTC249850.016.41619950-51
TATGATC216150.016.18476138-39
CGTCTTC267100.014.37823252-53
GCTATGA308150.014.05015936-37
GATCTCG245800.014.03922340-41
Produced by FastQC (version 0.11.5)