FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n01_ALm221RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n01_ALm221RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34157646
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT478020.13994524095717836TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC95600.032.4695346-47
TCGTATG95850.031.66636744-45
TATGCCG101750.030.15689848-49
CTCGTAT94750.028.75038744-45
TCTCGTA111150.023.1194442-43
CGGCTAT150350.021.07217634-35
CCGGCTA154250.020.27767832-33
ATCTCGT129150.019.78688242-43
ATGCCGT160100.019.10658648-49
TCACCGG173750.018.5350130-31
ACCGGCT177050.018.10904332-33
GTATGCC170300.018.05981646-47
GTCACCG186200.017.15543728-29
CCGTCTT171600.017.10678352-53
GCCGTCT170450.017.06860250-51
TATGATC170050.015.43281538-39
TGCCGTC201650.015.11079550-51
GCTATGA235400.013.43862236-37
CGTCTTC212150.013.344439552-53
GATCTCG195900.013.2993940-41
Produced by FastQC (version 0.11.5)