FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n01_ALm216RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n01_ALm216RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34410715
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG120500.030.70681234-35
AATGCGC145150.025.00118634-35
CGCGTAT16300.022.43843344-45
ATGCGCA179200.020.62176936-37
TGCGCAT169650.020.1587236-37
CGCATCG94750.019.57629838-39
ATCGCGT25800.019.51531442-43
GCGCATC172200.019.12943338-39
CTAATGC195350.018.85613632-33
GTCACTA218000.016.96250328-29
ACTAATG235000.016.15976132-33
TCGCGTA21700.015.8696642-43
CACTAAT241800.015.39106330-31
TCACTAA259800.014.92804530-31
TATGCCG46650.014.91671348-49
CGTATGC45200.014.08159846-47
TCGTATG38000.013.87486544-45
CTCGTAT48500.013.074615544-45
ACGTCTG298050.012.43908114-15
GAGCACA618750.012.3132919
Produced by FastQC (version 0.11.5)