FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n01_ALm215RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n01_ALm215RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32166708
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG114000.031.27027134-35
AATGCGC132800.026.32483134-35
CGCGTAT16550.024.82603344-45
ATCGCGT25600.023.19312542-43
ATGCGCA162950.021.87671936-37
TGCGCAT156250.021.14282236-37
CGCATCG85000.021.06740238-39
GCGCATC156550.019.75224538-39
CTAATGC185700.019.11987932-33
GTCACTA204500.017.46677628-29
TCGCGTA23400.017.15260342-43
ACTAATG218250.016.8015332-33
CACTAAT222200.016.07535630-31
TCACTAA241250.015.39665130-31
CGTATGC39150.015.1051446-47
TATGCCG46200.014.59936248-49
GAGCACA581750.012.71270759
ACGTCTG280400.012.67993914-15
CGTCTGA291950.012.54441216-17
CTCGTAT43550.012.27025444-45
Produced by FastQC (version 0.11.5)