FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n01_ALm214RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n01_ALm214RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36535858
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG129750.030.49470934-35
AATGCGC154750.024.98507334-35
CGCGTAT18900.024.37809644-45
ATCGCGT31250.021.20381742-43
ATGCGCA188850.020.91376136-37
TGCGCAT177750.020.60305836-37
CGCATCG98100.019.94884538-39
GCGCATC177550.019.7836838-39
CTAATGC211300.018.55688332-33
GTCACTA234450.016.82594928-29
ACTAATG251700.016.21997332-33
TCGCGTA26100.016.10618242-43
CACTAAT261300.015.26962130-31
CGTATGC46400.014.99707446-47
TCACTAA278450.014.97737330-31
TATGCCG52350.014.88038248-49
TCGTATG40850.012.6743144-45
ACGTCTG314950.012.42774814-15
GAGCACA665250.012.3880259
CGTCTGA332950.012.24811616-17
Produced by FastQC (version 0.11.5)