FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n01_ALm213RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n01_ALm213RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33460564
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG112200.029.274334-35
AATGCGC134200.023.83813534-35
CGCGTAT16000.020.33569744-45
ATCGCGT24950.019.4186142-43
ATGCGCA170400.019.26174536-37
TGCGCAT157000.019.02997436-37
GCGCATC155250.018.44905738-39
CGCATCG82600.018.31546238-39
CTAATGC186100.017.29223332-33
GTCACTA208450.015.91677128-29
TCGCGTA21050.015.56988642-43
ACTAATG224450.015.15240432-33
CACTAAT233150.014.13882130-31
TCACTAA251800.013.91218830-31
TATGCCG46350.013.62983848-49
CGTATGC41700.012.98543846-47
TACCGTA33450.012.0700759
TCGTATG36600.011.7450844-45
CTCGTAT43500.011.51997944-45
CGTCTGA300500.011.27850716-17
Produced by FastQC (version 0.11.5)