FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n01_ALm208RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n01_ALm208RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37869030
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT481450.1271355511350568TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG93650.029.72202944-45
CGTATGC97750.029.37422446-47
TATGCCG100200.028.49009548-49
CTCGTAT101450.026.87499844-45
TATCTCG112950.022.62482540-41
TCTCGTA124550.020.57484442-43
ATCTCGT142150.018.56205642-43
GTATGCC175700.016.24761446-47
ATGCCGT175150.016.20372648-49
GCTATCT162650.015.97431238-39
CCGTCTT178800.014.42558752-53
GCCGTCT177700.014.24725550-51
AGCTATC199050.013.69741438-39
CTATCTC206500.013.14574940-41
TGCCGTC227450.012.41530550-51
AAGCTAT251450.011.80633936-37
GAAGCTA269750.011.780169536-37
TACCGTA38250.010.927959
CGTCTTC231750.010.57623752-53
AGTCACC327250.010.1530628-29
Produced by FastQC (version 0.11.5)