FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n01_ALm207RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n01_ALm207RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36630626
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT445300.12156494404436333TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG86450.029.86242144-45
CGTATGC92150.028.91711646-47
TATGCCG96000.027.70798148-49
CTCGTAT94400.026.6179244-45
TATCTCG104650.023.17114840-41
TCTCGTA118400.020.35987542-43
ATCTCGT134100.018.4543942-43
GCTATCT152550.015.95777738-39
ATGCCGT166050.015.86173748-49
GTATGCC168900.015.72061746-47
GCCGTCT170650.014.26518550-51
CCGTCTT174650.014.21081952-53
AGCTATC182050.014.0372638-39
CTATCTC196200.012.91595340-41
TGCCGTC208150.012.81336550-51
TACCGTA38150.012.3261369
GAAGCTA253350.011.70840836-37
AAGCTAT243750.011.60441936-37
CTACCGT47900.010.8088248
CGTCTTC217900.010.594552-53
Produced by FastQC (version 0.11.5)