FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n01_ALm206RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n01_ALm206RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39208073
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT554700.14147596593181205TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG103800.031.25462544-45
CGTATGC107400.030.84803846-47
TATGCCG110900.029.6603648-49
CTCGTAT110800.027.97252744-45
TATCTCG118950.025.19739240-41
TCTCGTA136700.021.68238442-43
ATCTCGT155550.019.60450742-43
ATGCCGT182250.017.73571848-49
GCTATCT177300.017.15931538-39
GTATGCC193800.017.15662646-47
GCCGTCT185500.015.88865650-51
CCGTCTT196150.015.36529552-53
AGCTATC205800.015.36003838-39
CTATCTC218200.014.50895340-41
TGCCGTC232150.013.80074550-51
AAGCTAT274900.012.63935836-37
GAAGCTA293900.012.61417136-37
TACCGTA37250.012.4964089
CGTCTTC245750.011.79054552-53
AGTCACC357300.010.36262128-29
Produced by FastQC (version 0.11.5)