FastQCFastQC Report
Thu 3 Oct 2019
HFWLHBGXC_n01_ALm205RN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWLHBGXC_n01_ALm205RN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32133991
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT537280.16719989745438094TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG92600.032.88020744-45
CGTATGC98150.032.20657746-47
TATGCCG100850.030.99108748-49
CTCGTAT99100.029.54928244-45
TATCTCG107100.026.16680540-41
TCTCGTA118450.023.39880242-43
ATCTCGT129650.022.36665242-43
ATGCCGT158550.019.32325448-49
GCTATCT148850.019.29015238-39
GTATGCC168250.018.80208446-47
GCCGTCT161500.017.64694850-51
CCGTCTT165150.017.45865452-53
AGCTATC177300.016.81098638-39
CTATCTC182650.016.08452440-41
TGCCGTC202350.015.18769850-51
GAAGCTA245200.014.26721336-37
AAGCTAT226650.014.2193936-37
TACCGTA35600.013.7427429
CGTCTTC207600.013.29381152-53
AGTCACC304850.011.73263628-29
Produced by FastQC (version 0.11.5)