FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23521238
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC673920.28651553119780515TruSeq Adapter, Index 22 (97% over 37bp)
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATA360660.15333376585024988No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA337750.14359363227394747No Hit
TTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCAA324550.13798168276686798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG92700.056.1766246
CGTATGC97050.053.98492444
ATGCCGT103550.049.8170447
GTATGCC106150.049.5866645
TCGTATG107950.048.1136843
CTCGTAT125850.040.9660842
GCCGTCT124000.040.86752349
GGTAGCA147900.038.08728434
GTAGCAT161900.033.90686835
CATCTCG153450.033.80925839
TGCCGTC152350.033.74496548
CGGTAGC177000.031.98337433
GTCACGG191750.029.70177829
ACGGTAG194100.029.41806632
TCTCGTA176650.028.94888141
CCGTCTT178900.028.89341450
AGTCACG198400.028.79346828
AAAAGGG143700.028.03150270
CACGGTA218550.026.39770731
GCATCTC206800.025.32509238
Produced by FastQC (version 0.11.2)