FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23056759
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGC687730.29827696078186877TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG102150.055.53585446
CGTATGC106300.053.59863744
ATGCCGT111600.050.42555247
GTATGCC117850.048.64219345
TCGTATG120200.046.03381343
TGCCGTC125650.043.6727548
GCCGTCT127400.042.16633649
CGAGTGG153050.040.6519833
CTCGTAT137400.038.4399842
ACGAGTG172600.036.75686332
AGTGGAT166900.036.06170335
GAGTGGA183050.033.68350234
CACGAGT196050.032.5370431
GTCACGA214250.029.67280229
AGTCACG215000.029.53512428
GTGGATC194750.029.2326936
TCTCGTA178850.029.06333741
GATCTCG185100.028.38961439
AAAAGGG159100.028.19929170
CCGTCTT201500.027.35443350
Produced by FastQC (version 0.11.2)