FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22702286
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC1088800.47959927912105416TruSeq Adapter, Index 22 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC152350.058.04985444
TATGCCG153050.057.98913646
ATGCCGT160400.055.5281547
GTATGCC167450.052.91906445
TCGTATG169100.052.15653243
TGCCGTC172100.051.44788448
GCCGTCT178500.049.44629349
CGTACGA184550.046.7629634
GTACGAT188750.044.86926735
CTCGTAT188750.044.50379642
CCGTACG199400.044.28135733
TACGATC198250.041.09427636
ACCGTAC227550.039.04901532
TCTCGTA230250.035.9223441
ATCTCGT227700.035.4662140
GATCTCG235850.034.24297739
CCGTCTT261700.034.08725750
AAAAGGG186450.033.7106470
CTGCTTG271550.032.50261757
ACGATCT258550.031.5229637
Produced by FastQC (version 0.11.2)