Basic Statistics
Measure | Value |
---|---|
Filename | HFWHCBGXY_n01_shoot21.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 21585339 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC | 98513 | 0.4563884773827272 | TruSeq Adapter, Index 21 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 13395 | 0.0 | 59.203964 | 46 |
CGTATGC | 13415 | 0.0 | 59.116936 | 44 |
ATGCCGT | 14240 | 0.0 | 55.78873 | 47 |
TCGTATG | 14980 | 0.0 | 52.87307 | 43 |
GTATGCC | 15190 | 0.0 | 52.369724 | 45 |
TGCCGTC | 15700 | 0.0 | 50.46687 | 48 |
GCCGTCT | 16140 | 0.0 | 48.87412 | 49 |
CTCGTAT | 17185 | 0.0 | 43.198433 | 42 |
CGTTTCG | 18880 | 0.0 | 42.02034 | 33 |
GTTTCGA | 21660 | 0.0 | 35.819122 | 34 |
GATCTCG | 20005 | 0.0 | 35.698833 | 39 |
GTCACGT | 23000 | 0.0 | 34.778572 | 29 |
CACGTTT | 23150 | 0.0 | 34.40392 | 31 |
TTCGATC | 21015 | 0.0 | 34.15239 | 36 |
TCTCGTA | 21570 | 0.0 | 33.964333 | 41 |
TTTCGAT | 22835 | 0.0 | 33.531227 | 35 |
CCGTCTT | 24000 | 0.0 | 33.49464 | 50 |
ATCTCGT | 21795 | 0.0 | 33.02193 | 40 |
ACGTTTC | 24820 | 0.0 | 32.118694 | 32 |
AGTCACG | 24970 | 0.0 | 32.02004 | 28 |