FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21585339
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC985130.4563884773827272TruSeq Adapter, Index 21 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG133950.059.20396446
CGTATGC134150.059.11693644
ATGCCGT142400.055.7887347
TCGTATG149800.052.8730743
GTATGCC151900.052.36972445
TGCCGTC157000.050.4668748
GCCGTCT161400.048.8741249
CTCGTAT171850.043.19843342
CGTTTCG188800.042.0203433
GTTTCGA216600.035.81912234
GATCTCG200050.035.69883339
GTCACGT230000.034.77857229
CACGTTT231500.034.4039231
TTCGATC210150.034.1523936
TCTCGTA215700.033.96433341
TTTCGAT228350.033.53122735
CCGTCTT240000.033.4946450
ATCTCGT217950.033.0219340
ACGTTTC248200.032.11869432
AGTCACG249700.032.0200428