FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24462719
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC770310.314891406797421TruSeq Adapter, Index 20 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC126750.055.88558244
TATGCCG127800.054.87817446
ATGCCGT139100.049.89119747
TCGTATG145000.047.98447443
GTATGCC150750.047.54535745
TGCCGTC146350.046.77400248
GCCGTCT156900.042.66977749
CGTGGCC202000.041.99540333
ACGTGGC206150.041.31949232
CACGTGG213750.039.65210331
GTGGCCA221600.037.649234
CTCGTAT172850.036.1443742
GTCACGT238900.036.09072529
TCACGTG241250.035.37763630
GGCCATC197500.033.89354336
AGTCACG254400.033.7392628
CATCTCG186650.033.32869739
TGGCCAT253700.032.6508835
AAAAGGG194600.030.08693370
ATCTCGT207700.029.99937640
Produced by FastQC (version 0.11.2)