Basic Statistics
Measure | Value |
---|---|
Filename | HFWHCBGXY_n01_shoot19.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 24916610 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATGC | 172507 | 0.6923373604996828 | TruSeq Adapter, Index 19 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 22035 | 0.0 | 60.720703 | 44 |
TATGCCG | 22430 | 0.0 | 59.790977 | 46 |
ATGCCGT | 23250 | 0.0 | 57.124924 | 47 |
GTATGCC | 24080 | 0.0 | 55.99933 | 45 |
TCGTATG | 24035 | 0.0 | 55.363113 | 43 |
TGCCGTC | 23910 | 0.0 | 54.874657 | 48 |
GCCGTCT | 25305 | 0.0 | 51.047302 | 49 |
CTCGTAT | 27640 | 0.0 | 47.688625 | 42 |
ACGTGAA | 34045 | 0.0 | 43.47823 | 32 |
AATCTCG | 31230 | 0.0 | 42.741455 | 39 |
CGTGAAA | 34900 | 0.0 | 42.343365 | 33 |
ATCTCGT | 31865 | 0.0 | 41.732895 | 40 |
CACGTGA | 36255 | 0.0 | 40.71925 | 31 |
TCTCGTA | 32730 | 0.0 | 40.35943 | 41 |
AAAAGGG | 25960 | 0.0 | 39.850033 | 70 |
GTCACGT | 37700 | 0.0 | 39.46298 | 29 |
TCACGTG | 37955 | 0.0 | 38.89411 | 30 |
CCGTCTT | 35150 | 0.0 | 37.535965 | 50 |
AGTCACG | 40080 | 0.0 | 37.231815 | 28 |
CTGCTTG | 35440 | 0.0 | 36.37898 | 57 |