FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24916610
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATGC1725070.6923373604996828TruSeq Adapter, Index 19 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC220350.060.72070344
TATGCCG224300.059.79097746
ATGCCGT232500.057.12492447
GTATGCC240800.055.9993345
TCGTATG240350.055.36311343
TGCCGTC239100.054.87465748
GCCGTCT253050.051.04730249
CTCGTAT276400.047.68862542
ACGTGAA340450.043.4782332
AATCTCG312300.042.74145539
CGTGAAA349000.042.34336533
ATCTCGT318650.041.73289540
CACGTGA362550.040.7192531
TCTCGTA327300.040.3594341
AAAAGGG259600.039.85003370
GTCACGT377000.039.4629829
TCACGTG379550.038.8941130
CCGTCTT351500.037.53596550
AGTCACG400800.037.23181528
CTGCTTG354400.036.3789857