FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25509877
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGC812750.3186020849884929TruSeq Adapter, Index 18 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG113850.055.6087746
CGTATGC118500.053.27939244
ATGCCGT124350.050.65971847
GTATGCC130100.049.0129145
TCGTATG133300.046.6035243
GTCCGCA156450.044.0613434
CGTCCGC160700.043.37551533
GCCGTCT143750.042.31323649
TGCCGTC147600.042.18176748
CTCGTAT155300.039.641842
ACGTCCG185600.037.70666532
CGCATCT183600.034.0551437
TCCGCAT202850.033.74077635
CATCTCG192300.032.12978739
GTCACGT232650.030.574129
TCTCGTA201900.030.30292541
CCGTCTT211650.029.35036750
AAAAGGG169600.028.82713370
TCACGTC249350.028.66737630
CCGCATC235050.028.50766236
Produced by FastQC (version 0.11.2)