FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29036331
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC1607730.5536959886564181TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG207600.060.654446
CGTATGC213650.059.2819444
ATGCCGT222100.056.71008347
GTATGCC224950.056.24138345
TCGTATG233700.054.18234343
GCCGTCT247600.050.50209449
TGCCGTC250500.050.2526548
CTCGTAT251450.048.9674942
ACGTAGA323600.039.41283832
TCTCGTA312300.039.361941
CCGTCTT322650.039.199750
CGTAGAG327350.038.9298633
TCACGTA336400.037.9936930
GATCTCG322500.037.89446639
ATCTCGT337550.036.1298840
GTCACGT357800.035.89628629
CTGCTTG351300.035.6241257
AAAAGGG254950.035.4704570
AGTCACG387550.033.01331328
TGAAAAA405050.031.67468862