Basic Statistics
Measure | Value |
---|---|
Filename | HFWHCBGXY_n01_shoot17.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 29036331 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC | 160773 | 0.5536959886564181 | TruSeq Adapter, Index 3 (97% over 37bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 20760 | 0.0 | 60.6544 | 46 |
CGTATGC | 21365 | 0.0 | 59.28194 | 44 |
ATGCCGT | 22210 | 0.0 | 56.710083 | 47 |
GTATGCC | 22495 | 0.0 | 56.241383 | 45 |
TCGTATG | 23370 | 0.0 | 54.182343 | 43 |
GCCGTCT | 24760 | 0.0 | 50.502094 | 49 |
TGCCGTC | 25050 | 0.0 | 50.25265 | 48 |
CTCGTAT | 25145 | 0.0 | 48.96749 | 42 |
ACGTAGA | 32360 | 0.0 | 39.412838 | 32 |
TCTCGTA | 31230 | 0.0 | 39.3619 | 41 |
CCGTCTT | 32265 | 0.0 | 39.1997 | 50 |
CGTAGAG | 32735 | 0.0 | 38.92986 | 33 |
TCACGTA | 33640 | 0.0 | 37.99369 | 30 |
GATCTCG | 32250 | 0.0 | 37.894466 | 39 |
ATCTCGT | 33755 | 0.0 | 36.12988 | 40 |
GTCACGT | 35780 | 0.0 | 35.896286 | 29 |
CTGCTTG | 35130 | 0.0 | 35.62412 | 57 |
AAAAGGG | 25495 | 0.0 | 35.47045 | 70 |
AGTCACG | 38755 | 0.0 | 33.013313 | 28 |
TGAAAAA | 40505 | 0.0 | 31.674688 | 62 |