FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22879519
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC756290.330553277802737TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG118600.055.4761246
CGTATGC121050.054.47002844
ATGCCGT127000.051.44803647
GTATGCC135300.049.17257745
TCGTATG133350.048.24073843
TGCCGTC135750.047.1778948
GCCGTCT140450.044.7019149
CCCGTCC175600.041.9893933
CTCGTAT149700.040.63593742
CCGTCCA186550.039.07438334
ACCCGTC193200.038.10915432
CATCTCG181400.032.65286639
CACCCGT226900.032.54034831
CGTCCAT228750.030.90166735
TCTCGTA194150.030.59500141
TCACCCG242850.030.35870730
ATCTCGT197850.029.97153940
CCGTCTT217900.029.61611450
AAAAGGG169400.029.17068770
GTCACCC254300.029.14232429
Produced by FastQC (version 0.11.2)