FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22080846
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC1237550.5604631271827176TruSeq Adapter, Index 15 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG165050.060.3253946
CGTATGC169150.059.0918444
ATGCCGT176000.056.71100647
GTATGCC178050.056.17713545
TCGTATG185000.053.86104243
TGCCGTC191700.051.7379448
GCCGTCT193550.051.04464349
CTCGTAT202100.046.96714842
TCTCGTA251700.037.700241
CCGTCTT265000.037.69116250
GTCAATC258000.036.59796536
ATCTCGT260800.036.36012340
AATCTCG264050.035.8357339
AAAAGGG213550.034.56276370
CTGCTTG287250.034.3571157
ACATGTC306550.032.90271832
CACATGT311800.032.34614631
CATGTCA318850.031.63403133
GTCACAT323200.031.3221729
GCTTGAA319650.031.23586859
Produced by FastQC (version 0.11.2)