FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27002777
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC1105840.40952824963151013TruSeq Adapter, Index 14 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG158800.058.18151546
CGTATGC160850.057.9195344
ATGCCGT167450.054.8201147
GTATGCC179700.052.09663845
TCGTATG179150.051.43771743
TGCCGTC193000.046.9099648
GCCGTCT191950.046.3644449
CTCGTAT203350.041.79022642
CATCTCG228350.036.5023739
TCTCGTA258800.032.62230341
CCGTCTT283950.032.00775550
ATCTCGT266800.031.4892540
ACAGTTC325700.031.00099232
AAAAGGG234450.030.49602770
CTGCTTG307600.029.0230957
CACAGTT371800.027.3719931
GTCACAG376400.027.02642329
TGAAAAA366950.026.43700662
GCTTGAA354000.025.84198659
CAGTTCC395700.025.53503233
Produced by FastQC (version 0.11.2)