FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29763033
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC2155310.7241567080881844TruSeq Adapter, Index 13 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC284950.061.1149244
TATGCCG285900.060.6541746
ATGCCGT299950.057.83581547
GTATGCC307850.056.60249745
TCGTATG308050.056.56784443
TGCCGTC312000.055.3216748
GCCGTCT323250.053.0065749
CTCGTAT338300.048.93597442
AATCTCG394200.041.84622639
ATCTCGT395200.041.70126340
TCTCGTA401650.041.3069441
CCGTCTT437650.039.72660450
GTCAAAT442850.039.5292835
AAAAGGG322700.038.42342470
CTGCTTG450450.038.04579557
ACAGTCA470900.037.88020332
CAGTCAA489200.036.5211133
CACAGTC498650.035.88922531
TGAAAAA499650.035.42031562
GCTTGAA494300.034.94693859
Produced by FastQC (version 0.11.2)