FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20354389
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1226650.6026464365990057TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG150950.061.2068346
CGTATGC156400.059.25401344
ATGCCGT161300.057.4094647
GTATGCC164100.056.64357845
TCGTATG169700.054.63310243
TGCCGTC174800.052.79547548
GCCGTCT178000.051.4925749
CGGCTAC183200.051.0445433
ACGGCTA191100.049.29994232
CTCGTAT186250.049.29218342
GGCTACA192100.048.6439134
GCTACAT201100.046.20553235
CACGGCT209900.044.79839331
CATCTCG207450.043.8247939
CTACATC210300.043.75121736
GTCACGG226250.041.54310229
TACATCT223050.041.1714237
AGTCACG230700.040.70972428
TCACGGC232950.040.5445130
TCTCGTA230250.039.7687441
Produced by FastQC (version 0.11.2)