FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20209547
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC596560.2951872201786611TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG87850.056.84781646
CGTATGC91650.054.6065644
GTATGCC101800.050.2958845
ATGCCGT97250.049.91341847
TCGTATG102850.047.0617143
GCCGTCT108300.042.2677149
TGCCGTC112450.041.67249348
TATCTCG122050.041.24598339
CTCGTAT122000.039.7620142
TAGCTTA165300.039.2042234
GCTTATC166650.037.56235536
AGCTTAT184000.034.99128735
CACTAGC193800.033.29255331
ACTAGCT197250.032.85321432
TCACTAG202550.031.92213630
AAAAGGG139950.031.1331270
TCTCGTA162500.030.13402741
CTTATCT179950.029.98015637
GTCACTA219400.029.53345329
AGTCACT221650.029.16961328
Produced by FastQC (version 0.11.2)