FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21366482
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC2763301.2932873085985797TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC337000.063.59763744
TATGCCG336950.063.51270746
ATGCCGT346000.061.96272747
TCGTATG350650.061.0041643
GTATGCC352900.060.81145
TGCCGTC351900.060.76487448
GCCGTCT359600.059.36668849
CTCGTAT368450.056.38786342
CGATCAG391650.054.65421733
ATCTCGT396800.051.62509540
GATCTCG405300.050.38137439
TCTCGTA410550.050.26736541
GTCACGA430850.049.98372729
AGTCACG434250.049.75208328
ACGATCA440350.049.02264832
CCGTCTT443200.048.53959350
AAAAGGG317800.047.83740270
TCACGAT451800.047.7916230
CTGCTTG447100.047.6927257
CACGATC463050.046.6017931
Produced by FastQC (version 0.11.2)