FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31149203
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1860840.5973957022271164TruSeq Adapter, Index 8 (100% over 50bp)
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATA328450.10544411039987124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG243450.062.77729446
CGTATGC249550.060.8796144
ATGCCGT254150.058.55063647
GTATGCC272650.057.35100645
TCGTATG260000.056.02514643
GCCGTCT269750.051.14286449
TGCCGTC286400.050.05130448
CTCGTAT292350.049.6006142
GTCACAC461050.045.03931429
TCTCGTA364200.040.31686441
CACACTT519600.040.1755231
ACACTTG523150.039.6636532
AAAAGGG345000.039.30782370
CCGTCTT366250.038.83332450
CACTTGA537950.038.67705533
CAGTCAC549200.037.9607627
AATCTCG404800.037.89528339
TCACACT560750.037.30088430
ATCTCGT405050.037.1177840
AGTCACA574650.036.40870728
Produced by FastQC (version 0.11.2)