FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22339385
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC808920.3621048654651863TruSeq Adapter, Index 3 (100% over 50bp)
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATA237440.1062876171389678No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG110950.058.3860346
CGTATGC113150.057.3138344
ATGCCGT120250.053.4627147
GTATGCC128550.051.18226245
TCGTATG126900.050.30583243
GCCGTCT142250.043.57056849
TGCCGTC148500.042.5380648
CTCGTAT148250.042.14237642
TAGGCAT188100.038.24976735
CATCTCG174500.035.93049239
GGCATCT178100.035.8750237
AGGCATC195200.035.20784836
TTAGGCA211950.034.77158734
TCTCGTA196850.031.58036241
ACTTAGG237150.031.12028332
CCGTCTT209000.030.62621750
CACTTAG245100.030.18080931
AAAAGGG171250.030.10192170
TCACTTA270150.027.87363430
ATCTCGT224850.027.8676840
Produced by FastQC (version 0.11.2)