FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22340981
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1298040.581012982375304TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC170550.058.7097744
TATGCCG174150.057.41433346
ATGCCGT179900.055.71504647
TCGTATG187800.053.39283843
TGCCGTC191000.052.3855448
GTATGCC192100.052.2136845
GCCGTCT200750.049.7365849
CTCGTAT220400.043.8776842
GATCTCG243350.038.38083339
ATCTCGT245150.038.31073840
TCTCGTA253650.037.5622841
TCACGAT268800.036.54713435
AAAAGGG202300.035.72340470
CACGATC266350.035.0167236
CCGTCTT296550.034.3654650
ACGATCT275150.034.07447437
ATCACGA296800.033.9133634
CATCACG302050.033.79913333
CTGCTTG307600.032.67547657
CGATCTC289550.032.54849238
Produced by FastQC (version 0.11.2)