FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20786680
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1858360.8940148210296209TruSeq Adapter, Index 12 (100% over 50bp)
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATA305300.14687290130025574No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA277230.13336906134120502No Hit
TTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCAA214710.10329210821545336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG224500.063.89935746
CGTATGC230100.062.40536544
ATGCCGT231650.061.2463347
GTATGCC239200.060.41165545
TCGTATG240650.059.74440843
GCCGTCT242400.057.18760749
CTCGTAT245550.054.84942
TGCCGTC272550.051.6703648
CCGTCTT294250.047.57427250
TCTCGTA294050.045.82898741
GTCACCT337850.045.36135529
GTAATCT301600.045.35188737
AAAAGGG237500.044.88413270
TGTAATC316550.043.75256336
TTGTAAT349250.043.6258735
CTGCTTG316100.043.44360457
AGTCACC359050.043.12048328
TAATCTC327400.041.74525538
CCTTGTA369400.041.68243433
ATCTCGT330300.040.96103740