Basic Statistics
Measure | Value |
---|---|
Filename | HFWHCBGXY_n01_shoot06.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 20786680 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC | 185836 | 0.8940148210296209 | TruSeq Adapter, Index 12 (100% over 50bp) |
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATA | 30530 | 0.14687290130025574 | No Hit |
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA | 27723 | 0.13336906134120502 | No Hit |
TTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCAA | 21471 | 0.10329210821545336 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 22450 | 0.0 | 63.899357 | 46 |
CGTATGC | 23010 | 0.0 | 62.405365 | 44 |
ATGCCGT | 23165 | 0.0 | 61.24633 | 47 |
GTATGCC | 23920 | 0.0 | 60.411655 | 45 |
TCGTATG | 24065 | 0.0 | 59.744408 | 43 |
GCCGTCT | 24240 | 0.0 | 57.187607 | 49 |
CTCGTAT | 24555 | 0.0 | 54.849 | 42 |
TGCCGTC | 27255 | 0.0 | 51.67036 | 48 |
CCGTCTT | 29425 | 0.0 | 47.574272 | 50 |
TCTCGTA | 29405 | 0.0 | 45.828987 | 41 |
GTCACCT | 33785 | 0.0 | 45.361355 | 29 |
GTAATCT | 30160 | 0.0 | 45.351887 | 37 |
AAAAGGG | 23750 | 0.0 | 44.884132 | 70 |
TGTAATC | 31655 | 0.0 | 43.752563 | 36 |
TTGTAAT | 34925 | 0.0 | 43.62587 | 35 |
CTGCTTG | 31610 | 0.0 | 43.443604 | 57 |
AGTCACC | 35905 | 0.0 | 43.120483 | 28 |
TAATCTC | 32740 | 0.0 | 41.745255 | 38 |
CCTTGTA | 36940 | 0.0 | 41.682434 | 33 |
ATCTCGT | 33030 | 0.0 | 40.961037 | 40 |