FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21899702
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1401980.6401822271371547TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC187500.060.14002244
TATGCCG188500.059.50440246
ATGCCGT191850.057.51664747
TCGTATG195150.055.704643
GTATGCC205750.055.62170445
TGCCGTC198350.054.7847748
GCCGTCT201350.052.56075349
CTCGTAT219550.048.70334242
CATCTCG246550.044.4707239
AAAAGGG221750.041.19109370
ATCTCGT265550.041.02200740
TCTCGTA265600.040.2347741
CAGATCA349800.038.53380634
CCGTCTT286050.037.6701350
AGTCACC367500.036.96697228
CTGCTTG288200.035.8463457
AAAAAGG258450.035.5451469
TGAAAAA347700.035.2282362
AGATCAT386450.034.64348635
ACCAGAT395950.034.51042632
Produced by FastQC (version 0.11.2)