FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22626307
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2150630.9504997877028717TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC255500.062.6811644
TATGCCG257750.061.83458746
ATGCCGT263550.060.39396747
TCGTATG270600.059.34112543
GTATGCC270650.059.19822745
TGCCGTC275400.057.642748
GCCGTCT283300.055.89916249
TATCTCG288750.055.19773539
CTCGTAT298500.053.3508842
CGCCAAT322000.050.2310233
GCCAATA324600.049.8504734
GTCACGC328550.049.1492629
CACGCCA333600.048.4398331
ACGCCAA335250.048.29786332
TCACGCC335900.048.06476230
TCTCGTA344750.046.43960641
ATCTCGT345100.046.4154640
CCAATAT358750.045.38762335
CCGTCTT363350.043.9305150
AGTCACG370850.043.7398628
Produced by FastQC (version 0.11.2)