Basic Statistics
Measure | Value |
---|---|
Filename | HFWHCBGXY_n01_shoot03.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 22863540 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC | 118094 | 0.5165166898914166 | TruSeq Adapter, Index 5 (100% over 50bp) |
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATA | 29875 | 0.1306665546980039 | No Hit |
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA | 26498 | 0.1158963135192538 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC | 24203 | 0.10585849785291343 | TruSeq Adapter, Index 5 (98% over 50bp) |
TTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCAA | 22902 | 0.10016821542070914 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 17815 | 0.0 | 61.822193 | 46 |
CGTATGC | 17965 | 0.0 | 61.52096 | 44 |
ATGCCGT | 18350 | 0.0 | 58.951584 | 47 |
GTATGCC | 19445 | 0.0 | 57.43191 | 45 |
TCGTATG | 19050 | 0.0 | 56.015507 | 43 |
GCCGTCT | 19985 | 0.0 | 52.009644 | 49 |
TGCCGTC | 22045 | 0.0 | 48.102005 | 48 |
CTCGTAT | 18515 | 0.0 | 47.674923 | 42 |
CACAGTG | 29250 | 0.0 | 43.484123 | 33 |
CACACAG | 30245 | 0.0 | 42.35232 | 31 |
ACACAGT | 30370 | 0.0 | 42.110313 | 32 |
GATCTCG | 22085 | 0.0 | 39.976227 | 39 |
CCGTCTT | 26560 | 0.0 | 39.85897 | 50 |
GTCACAC | 32270 | 0.0 | 39.56177 | 29 |
AAAAGGG | 22665 | 0.0 | 38.61773 | 70 |
AGTGATC | 24335 | 0.0 | 38.49869 | 36 |
CAGTGAT | 32140 | 0.0 | 38.452095 | 35 |
ACAGTGA | 32665 | 0.0 | 38.391666 | 34 |
TCTCGTA | 23325 | 0.0 | 37.710663 | 41 |
GTGATCT | 24895 | 0.0 | 36.39426 | 37 |