FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22863540
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1180940.5165166898914166TruSeq Adapter, Index 5 (100% over 50bp)
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATA298750.1306665546980039No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA264980.1158963135192538No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC242030.10585849785291343TruSeq Adapter, Index 5 (98% over 50bp)
TTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCAA229020.10016821542070914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG178150.061.82219346
CGTATGC179650.061.5209644
ATGCCGT183500.058.95158447
GTATGCC194450.057.4319145
TCGTATG190500.056.01550743
GCCGTCT199850.052.00964449
TGCCGTC220450.048.10200548
CTCGTAT185150.047.67492342
CACAGTG292500.043.48412333
CACACAG302450.042.3523231
ACACAGT303700.042.11031332
GATCTCG220850.039.97622739
CCGTCTT265600.039.8589750
GTCACAC322700.039.5617729
AAAAGGG226650.038.6177370
AGTGATC243350.038.4986936
CAGTGAT321400.038.45209535
ACAGTGA326650.038.39166634
TCTCGTA233250.037.71066341
GTGATCT248950.036.3942637